Responsibilities

Job Responsibilities

• Grow, maintain and prepare specialised suspension and adherent cell lines (epithelial) to be used in assay work.

• Maintain a frozen cell supply as required.

• Prepare conditioned media for successful maintenance of cells.

• Use of flourescent microscopy for imaging & cell counting

• Supply cells for ad-hoc and routine requests within established timelines.

• Co-ordinate the regular supply of consumables to ensure smooth delivery of cells to biological groups.

• Ensure aseptic technique is adhered to in the maintenance of cells and compliance to Health and Safety procedures for own well-being and quality of final product.

• Actively seek opportunities to develop the service by discussion with customer or by the sharing of best practice with other groups.

• Be an active member of the scientific teams – contribute at team meetings and look for opportunities for innovation.

• Assist with the preparation of SOPs, Risk Assessments and best practice guides.

• To act as an ambassador for TLS and to promote PerkinElmer’s core values.

• Operate to approved quality standards.

• Work independently and flexibly depending on team priorities and to set realistic work schedules to support requirements.

Desirable skills

• Good understanding and previous experience of working with epithelial cultures

• Experience in cell differentiation

• Experience in a number of technical analytical procedures, e.g., ELISA, MSD immunoplexes, Flow Cytometry and qPCR

• Data analysis skills (GraphPad, Prism, FlowJo, Excel)

• Experience of working in an industrial environment

Education/Experience/Qualifications

• BSc in a relevant scientific subject (e.g. biology, biotechnology or Biomedical Sciences), with relevant laboratory experience.

• Demonstrable knowledge of cell culture, cell lines, primary cells and aseptic technique.

• Demonstrable experience of working in a laboratory environment with experience of media preparation, cell isolation techniques and molecular biology methods.

• Understanding of standard software packages (i.e., Microsoft Office).

• Maintain an electronic laboratory notebook and present at team/project meetings.

• Highly motivated and independent, yet able to work well in a group setting.

• Ability to deliver high-quality, consistent results in a timely fashion.

• Ability to meet timelines successfully with the utmost attention to detail while setting high performance standards.

• Ability to understand, interpret, and communicate complex scientific data.

• Experience in design and execution of new and established methods and protocols.

Physical Demands 

The overall physical exertion of this position requires:

• Sit or stand for 4-5 hours in laboratory areas.

• Work around moving laboratory equipment and machinery.

• Work with biohazards such as blood borne pathogens.

• All work is carried out in a containment level 2 environment.

• Appropriate PPE must always be worn (Lab coat, gloves, eye protection).

• Reach above/below the shoulder, bend at the knees and waist.

• Office work activities, including keyboard, up to 2-3 hours.

 Working Environment

• Must be able to work in a laboratory, controlled environment requiring personal protective equipment.

• Job pace may be fast and job completion demands may be high.

• Employee will be required to manage hazardous waste in compliance with company procedures & State/Federal/Local hazardous waste regulations. Duties may include: identifying, handling, generating, accumulating, storing and labelling.

• Maintain a high standard work environment by ensuring a high level of safety and security is met in all aspects of daily activities, and ensure any potential safety hazards are addressed and corrected immediately.

• Understand ergonomic relationship between people, equipment and working environment.