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Biochemist - Bacterial Cell Culture

San Diego, California

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Location San Diego, CaliforniaUS Job ID JR-029452

BioLegend, Inc. (a PerkinElmer Company) develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA. Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support. Our product expertise covers a diverse set of research areas including Immunology, Neuroscience, Cancer, Stem cells, and Cell Biology.

In 2021, PerkinElmer, Inc. (NYSE: PKI) a global leader committed to innovating for a healthier world, acquired BioLegend. The acquisition, the largest in PerkinElmer’s history, further expands the Company’s life science franchise into high-growth areas such as cytometry, proteogenomics, multiplex assays, recombinant proteins, magnetic cell separation and bioprocessing.

Job Summary

This position is responsible for manufacturing cutting-edge recombinant protein research products in Molecular Cellular Immunology (MCI) group. The recombinant manufacturing associate will be involved in different stages of production of various recombinant protein products, which includes recombinant protein expression and purification. The duties include preparing buffer solutions and cell culture media, performing bacterial cultures, purifying recombinant proteins, and performing DNA isolation according to standard operating procedures. Another part of the job responsibility includes transferring the products to other groups for testing, preparing product/project-related documentation, and maintaining the inventory and record for recombinant protein products.

Essential Functions

Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

  • Prepare various buffers for experiments, including cell culture media.

  • Perform transformation and culture of bacterial cells

  • Process the culture supernatant/cell pellet/inclusion body, followed by extensive processing of the supernatant/cell pellet/inclusion body or diafiltration to extract raw protein bulk

  • Purify recombinant protein by means of affinity tag

  • Monitor processing by SDS PAGE for varied protein production stages

  • Perform bulk processing (concentration, dilution, buffer change), purity analysis, and endotoxin removal

  • Support inventory management of raw material and plasmid constructs for protein production

  • Assist in transferring products across different departments for QC or other inventory-related activities

  • Maintain batch records and other documentation that are essential to be in compliance with ISO13485:2016

  • Maintain laboratory equipment and manage supplies

  • Other projects or responsibilities as may be required

iACT Competencies

  • innovate – Be better, keep improving, be more efficient, creative, and be in the forefront of development

  • Aspire – Have desire, purpose, and ambition challenging ourselves to push the limits and reach new heights

  • Collaborate – Foster teamwork, common goals, selflessness, communication, and mutual support

  • Transform – Adapt, learn, re-invent and change for future development and growth

Minimum Qualifications Education and Experience

Associate’s Degree in Life Science or related area with at least 2 years of working experience in related biotech product development or manufacturing

Demonstrated ability to be detail-oriented, organized and work well in small workgroups

Able to perform basic computer operations such as MS Office and Outlook and other computer-based software

Preferred Qualifications – Education and Experience

Bachelor of Science in Life Science or related area

Experience in suspended E. coli culture

Experienced in purification and formulation of recombinant protein or antibody

Experienced in Product development or manufacturing under ISO/GMP environment

Work Environment & Physical Demands


While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job.

While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.

Condition of Employment

Concerns regarding COVID-19 continue as positive cases are experienced in the communities in which we operate. Please be aware that, as a condition of employment, BioLegend requires proof of COVID vaccination for all U.S.-based employees (subject to limited exceptions) beginning January 18, 2022.

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PerkinElmer is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity or expression, sexual orientation, national origin, genetics, disability status, age, or veteran status or any other characteristics protected by applicable law. PerkinElmer is committed to a culturally diverse workforce.

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